siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using pHR-SFFV-dCas9-BFP-KRAB to perform CRISPR Human - Repression lncRNA PVT1

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human plasma

Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric - human saliva

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hESC-EC

Get tips on using Anti-acetyl-Histone H3 Antibody to perform ChIP acH3 - Rabbit Human YFP

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using E-Cadherin (24E10) Rabbit mAb #3195 to perform Immunohistochemistry Human - E-Cadherin