rna-isolation-purification-cells-immortalized-brl-3a

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MCF-7

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - SH-SY5Y

Get tips on using LifeGuard Soil Preservation (1000 ml) to perform RNA stabilization Microbial

Get tips on using pET28a-bops to perform Protein Expression Prokaryotic cells - E. coli BRs

Get tips on using pET22b-bops to perform Protein Expression Prokaryotic cells - E. coli BRs

Get tips on using Blue Prestained Protein Standard, Broad Range (11-250 kDa) to perform Protein Ladder Prestained

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Blue Prestained Protein Marker, Broad Range (11-250 kDa) #59329 to perform Protein Ladder Prestained

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HeLa Lipofectamine

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - HESC Lipofectamine