Immunohistochemistry Anti-mouse IgG Donkey Mouse

Get tips on using Mouse CD200 R3 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - CD200

Get tips on using p-Chk2 (Thr 68)-R Antibody, rabbit polyclonal to perform Immunohistochemistry chk2 phospho (Thr 68) - Rabbit IgG Human -NA-

Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-

Get tips on using Mouse Sca-1/Ly6 APC-conjugated Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using Mouse IGF1 ELISA Kit (ab100695) to perform ELISA Mouse - IGF-I

Get tips on using BrdU antibody to perform Immunohistochemistry Mouse - BrdU

Get tips on using LC3B Antibody to perform Immunohistochemistry Mouse - LC3

Get tips on using LC3B Antibody to perform Immunohistochemistry Mouse - LC3

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.