Protein isolation Tissue

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Mycobacterium smegmatis

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Pectobacterium carotovorum

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Pseudomonas aeruginosa

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Staphylococcus aureus

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Pineal gland

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Liver

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Liver