Site Directed Mutagenesis (SDM) Human Deletion HepG2

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - HepG2 Fibrinogen

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - HepG2

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - HepG2

Get tips on using PowerPlex® 16 System to perform Cell line authentication Liver carcinoma cell line HepG2

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - HepG2

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - HepG2

Get tips on using Cultrex® BME Cell Invasion Assay to perform Cell migration / Invasion cell type - HepG2

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Autophagy assay cell type - HepG2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.