siRNA / miRNA gene silencing Mouse mESC

Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I

Get tips on using Phospho-ULK1 (Ser757) Antibody to perform Autophagy assay cell type - Human primary MSCs

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - rat MSC

Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Human primary MSCs

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC

Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - rat MSC

Get tips on using pLX-sgRNA to perform CRISPR Human - Deletion CIITA

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.