Site Directed Mutagenesis (SDM) Human Point mutation PC-3

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using Promega PCR Master Mix to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using QIAGEN Multiplex PCR Kit to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using Midnight RT PCR Expansion to perform PCR Conventional / Qualitative PCR - Viral DNA

Get tips on using Promega PCR Master Mix to perform PCR Conventional / Qualitative PCR - bacterial DNA

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using QuantiTect Multiplex PCR Kit (1000) to perform PCR Multiplex PCR - Bacterial DNA

Get tips on using QuantiFast Multiplex PCR Kit to perform PCR Quantitative real-time PCR - Bacterial DNA