Microarray Gene expression arrays Rat pancreas tissue

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - Rat_Liver

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Get tips on using PyroMark CpG Assays to perform DNA methylation profiling Whole genome profiling - human whole blood

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - RAW 264.7

Get tips on using GeneJET RNA Purification Kit to perform AAA for reviews

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Piscirickettsia salmonis

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Proteus mirabilis

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Lactobacillus brevis