siRNA / miRNA gene silencing Mouse B16-BL6

Get tips on using HER2/ErbB2 (29D8) Rabbit mAb #2165 to perform Western blotting HER2

Get tips on using p53 Antibody (DO-1): sc-126 to perform Western blotting p53

Get tips on using Anti-EGFR (phospho Y1068) antibody (ab5644) to perform Western blotting EGFR

Get tips on using EGFR Antibody (E-8): sc-374607 to perform Western blotting EGFR

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human blood endothelial cell

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood

Get tips on using Quantifiler™ Duo DNA Quantification Kit to perform DNA quantification Blood

Get tips on using Quantifiler 1 Trio DNA Quantification Kit to perform DNA quantification Blood

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.