rna-isolation-purification-cells-immortalized-ags

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - GH3

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - C2C12

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - rat nucleus pulposus

Get tips on using miRCURY LNA™ microRNA Power Labeling Kits to perform Microarray RNA amplification & Labeling - Rat spinal cord Hy5

Get tips on using ATP Assay to perform Cell cytotoxicity / Proliferation assay cell type - adipose stem cells

Get tips on using Guava Nexin Annexin V Assay to perform Apoptosis assay cell type - HeLa cells

Get tips on using Cellular Senescence Flow Cytometry Assay to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.