Microarray Gene expression arrays Mouse liver tissue

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Mouse - Lung adenocarcenoma (A549/LTEP-a-2) Lipofectamine

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Mouse - Point mutation 3T3-L1 Clk1

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)

Get tips on using Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) to perform Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) - Mouse Human -NA-

Get tips on using CD279 (PD-1) Monoclonal Antibody (J43), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Get tips on using CD273 (B7-DC) Monoclonal Antibody (TY25), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD273/PD-L2

Get tips on using CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1