Immunohistochemistry Alpha smooth muscle Actin Rabbit Mouse

Get tips on using GFAP (GA5) Mouse mAb #3670 to perform Immunohistochemistry Rat - GFAP

Get tips on using Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 to perform Western blotting NF-kB

Get tips on using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 to perform ChIP Anti-bodies H3K27me3

Get tips on using Mono-Methyl-Histone H3 (Lys27) (D3R8N) Rabbit mAb #84932 to perform ChIP Anti-bodies H3K27me1

Get tips on using Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 to perform ChIP Anti-bodies H3K4me2

Get tips on using Anti-PI3-kinase p85-α antibody produced in rabbit to perform Autophagy assay cell type - HepG2

Get tips on using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 to perform Autophagy assay cell type - HEK 293

Get tips on using Purified Mouse Anti-SV40 Large T Antigen Clone PAb 101 (RUO) to perform Immunohistochemistry Mouse - SV40

Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal to perform ChIP Anti-bodies H3K4me3

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.