Site Directed Mutagenesis (SDM) Mouse Deletion L929

Get tips on using Mouse/Rat CD34 Antibody to perform Immunohistochemistry Mouse - CD34

Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

Get tips on using Mouse Prolactin DuoSet ELISA to perform ELISA Mouse - PRL

Get tips on using Mouse Myeloperoxidase DuoSet ELISA to perform ELISA Mouse - MPO

Get tips on using Mouse Leptin ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse Decorin ELISA Kit to perform ELISA Mouse - Decorin

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .

Get tips on using ON-TARGETplus Mouse Stat5b (20851) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - FL83B Stat5b