Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.
Get tips on using RAB8A siRNA to perform siRNA / miRNA gene silencing Human - HEK293 Rab8a
Get tips on using ON-TARGETplus Human CDK1 (983) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK1
Get tips on using ON-TARGETplus Human CDK2 (1017) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK2
Get tips on using ON-TARGETplus Human SLC7A2 (6542) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC7A2
Get tips on using ON-TARGETplus Human SLC4A2 (6522) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 SLC4A2
Get tips on using ON-TARGETplus Human BEST1 (7439) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 BEST1
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