DNA methylation profiling Gene specific profiling MRC-5

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized 59M

Get tips on using pET28a-FSHR to perform Protein Expression Prokaryotic cells - E. coli FSHR-57aa proteins

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Detroit 562

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized RIN-5F

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized RIN-5F

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized K-562

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized K-562