rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using ChargeSwitch PCR Clean-Up Kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using Champion™ pET SUMO Expression System to perform Protein expression and purification Bacteria - Escherichia coli IFNA2

Get tips on using Gateway™ pDONR™221 Vector to perform Protein expression and purification Yeast - Saccharomyces cerevisiae ΔHSPA5

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris hmPRα

Get tips on using Ni-NTA Superflow 96 BioRobot Kit (4) to perform Protein tag Purification of His-tagged proteins

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using pgMAX system-rabbit voltage-dependent calcium channel β2a subunit to perform Protein Expression Prokaryotic cells - E. coli rabbit voltage-dependent calcium channel β2a subunit

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation E. coli DH5α