siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human BEAS2B

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human A549

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human HUVEC

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Astrocyte Medium to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - A2780

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - K562

Get tips on using EZ1 RNA Tissue Mini Kit (48) to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using InviTrap Spin Tissue RNA Mini Kit to perform RNA isolation / purification Tissue - Human Artery / Aorta

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - human breast tissue