Get tips on using Zombie Aqua™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - human peripheral blood mononuclear cells
I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using Anti-Apg7 antibody [EPR6251] to perform Autophagy assay cell type - SH-SY5Y
Get tips on using CnT-Prime, Epithelial Culture Medium to perform Mammalian cell culture media HEK
Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes
Get tips on using Cas9 sgRNA vector to perform CRISPR Human - Activation ESR1
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