RNA isolation / purification Cells Cancer cell lines

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - A2780

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U87

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - Jurkat

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - K562

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SKOV3

Get tips on using StemSpan™ SFEM to perform Stem cell Differentiation media hiPSCs differentiation into mesodermal lineage cells

Get tips on using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® to perform RNA sequencing Mouse - Bone marrow-derived macrophages (BMDMs)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.