siRNA / miRNA gene silencing Rat Schwann cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Get tips on using pTSara-NatB to perform Protein Expression Prokaryotic cells - E. coli N-terminal acetyltransferase B

Get tips on using pQE-30 to perform Protein Expression Prokaryotic cells - E. coli Guinea Pig TNF-Alpha

Get tips on using pET30a-β4 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus β4 tubulin

Get tips on using pET30a-α9 to perform Protein Expression Prokaryotic cells - E. coli E. granulosus α9 tubulin

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6

Get tips on using pPICZαC-MRP4-his6 to perform Protein Expression Eukaryotic cells - P. pastoris human MRP4-his6

Get tips on using pSS2-GLURP- SERA5 to perform Protein Expression Prokaryotic cells - L. lactis SERA5 P. falciparum

Get tips on using pSS2-GLURP- MSPDBL2 to perform Protein Expression Prokaryotic cells - L. lactis MSPDBL2 P. falciparum