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2 years ago
2 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 3 matching solutions for this experiment
|Seed 10,000 cells in 96-well plate.
Fix sample in 4% formaldehyde for 1 h before freezing.
|Fix sample in 4% formaldehyde for 5 min before using sample.
Block samples in 10% donkey serum in 0.1% Triton-X for 30 min at room temperature.
Incubate primary antibody overnight at 4 °C and with secondary antibodies for 1 h at room temperature