ELISA (kit) Human Serum Cytokine measurements (Multiplex assay)

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Get tips on using RevertAid RT Reverse Transcription Kit to perform cDNA synthesis Cell lines

Products Thermo Fisher Scientific RevertAid RT Reverse Transcription Kit

Get tips on using RNeasy 96 Universal Tissue Kit (4) to perform RNA isolation / purification Tissue - Mouse Kidney

Products Qiagen RNeasy 96 Universal Tissue Kit (4)

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Products Thermo Fisher Scientific PureLink™ FFPE RNA Isolation Kit

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Products Roche Lifesciences High Pure FFPET RNA Isolation Kit

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

RNA siRNA / miRNA gene silencing Human Aortic smooth muscle cell TSP-2

Get tips on using EpiTect Fast Bisulfite Kit (10) to perform Bisulfite DNA Modification FFPE samples

Products Qiagen EpiTect Fast Bisulfite Kit (10)

Get tips on using TurboCapture 96 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Products Qiagen TurboCapture 96 mRNA Kit (5)

Get tips on using TurboCapture 384 mRNA Kit (5) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Products Qiagen TurboCapture 384 mRNA Kit (5)

Get tips on using DyeEx 2.0 Spin Kit (250) to perform Removal of unincorporated dye terminators

Products Qiagen DyeEx 2.0 Spin Kit (250)

Get tips on using DNeasy Plant Mini Kit (250) to perform DNA isolation / purification Plants - Fungus

Products Qiagen DNeasy Plant Mini Kit (250)

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