DNA gel extraction / PCR product purification

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - TIG-118

Get tips on using Cytotoxicity Detection Kit (LDH) to perform Live / Dead assay mammalian cells - RAW 264.7

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Listeria innocua

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Staphylococcus epidermidis

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - rat nucleus pulposus

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - HepaRG human hepatoma

Get tips on using siGENOME Human DAB2 (1601) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 DAB2

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - INS-1 832/12

Get tips on using MultiTox-Fluor Multiplex Cytotoxicity Assay to perform Live / Dead assay mammalian cells - RAW 264.7