siRNA / miRNA gene silencing Mouse Pancreatic Acinar cells

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized A253

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized CMT12

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - CHO-K1

Get tips on using Nuclear Extract Kit to perform Protein isolation Mammalian cells - HOG

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Huh7

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.