DNA gel extraction / PCR product purification

Get tips on using Cell Counting Kit-8 (CCK-8) to perform Live / Dead assay mammalian cells - MMQ

Get tips on using Zombie Aqua™ Fixable Viability Kit to perform Live / Dead assay bacteria - Staphylococcus aureus

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - Spleen cells

Get tips on using Calcein AM Cell Viability Assay Kit to perform Live / Dead assay mammalian cells - 3T3-L1

Get tips on using Zombie Violet™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse microglia

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using Zombie Fixable Viability™ Sampler Kit to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Live / Dead assay mammalian cells - CHO-K1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using SYTO™ 9 Green Fluorescent Nucleic Acid Stain to perform Live / Dead assay bacteria - Pseudomonas aeruginosa