RNA sequencing Human

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - HeLa

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Liver cancer cell lines Hepato cellular carcenoma (SMMC-7721, Huh7 & HepG2))

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - BxPc-3 human primary pancreatic adenocarcinoma

Get tips on using miRCURY LNA™ microRNA Power Labeling Kits to perform Microarray RNA amplification & Labeling - Rat spinal cord Hy5

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Get tips on using QIAGEN Proteinase K (10 ml) to perform RNA isolation / purification Cells - primary mouse morula cells

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using PureLink ™ miRNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Get tips on using QIAamp MinElute Virus Vacuum Kit (50) to perform RNA isolation / purification Viral - Viral CNS disease