shRNA gene silencing Human HEK 293T

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Get tips on using pFRT to perform Protein Expression Eukaryotic cells - HeLa R19 cMBL

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HepG2

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using AcP(−)p6.9hEPO to perform Protein Expression Eukaryotic cells - S. frugiperda hEPO

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HepG2