rna-isolation-purification-tissue-mouse-spleen

Get tips on using HiSpeed Plasmid Midi Kit (25) to perform Plasmid Isolation E. coli

Get tips on using E.Z.N.A.® Plasmid Midi Kit to perform Plasmid Isolation Yersinia enterocolitica

Get tips on using TIANpure Mini Plasmid Kit(DP104) to perform Plasmid Isolation Citrobacter freundii

Get tips on using PureYield™ Plasmid Midiprep System to perform Plasmid Isolation Citrobacter freundii

Get tips on using PureYield™ Plasmid Midiprep System to perform Plasmid Isolation Streptococcus pneumoniae

Get tips on using Plasmid Miniprep Kit I, peqGOLD to perform Plasmid Isolation Staphylococcus aureus

Get tips on using GenElute™ Plasmid Miniprep Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.