Protein expression and purification Yeast Pichia pastoris

Get tips on using pGEX-6P-1 Vector to perform Protein expression and purification Bacteria - Escherichia coli FleN

Get tips on using pQE-30 Xa Vector to perform Protein expression and purification Bacteria - Escherichia coli Siva1

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Get tips on using Freedom™ DG44 Kit to perform Protein expression and purification Mammalian cells - CHO-K1 G12

Get tips on using pRSH to perform Protein Expression Eukaryotic cells - S. pastorianus cellulase

Get tips on using FreeStyle™ 293-F Cells to perform Protein expression and purification Mammalian cells - HEK 293 EGFR

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Bacteria - Gram positive Pseudomonas

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF