Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using Mouse heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Mouse - HO-1

Get tips on using Rat KIM-1 TIM-1 ELISA Kit (ab119597) to perform ELISA Rat - KIM-1

Get tips on using Rat heme oxygenase 1,HO-1 ELISA Kit to perform ELISA Rat - HO-1

Get tips on using CREB-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC CREB

Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF MEK1

Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1

Get tips on using ENX-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HaCaT EZH2

Get tips on using Flotillin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 FLOT1

Get tips on using ON-TARGETplus Human LYVE1 (10894) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 LYVE-1

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.