rna-isolation-purification-cells-immortalized-bxpc-3

Get tips on using EasySep™ Human B Cell Enrichment Kit II Without CD43 Depletion to perform Cell Isolation B cell

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Get tips on using Targefect-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using jetPEI®-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using FuGENE® 6 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human lung fibroblasts (HLF)

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?