siRNA / RNAi /miRNA transfection Human Cells KG-1

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human HUVEC

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - OVCAR-5

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - MCF-7

Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SH-SY5Y

Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - SW480 human colon cancer cell line

Get tips on using Neural Maintenance-XF Medium to perform 3D Cell Culture Media Human blood-brain barrier organoid

Get tips on using Gibco™ DMEM, high glucose to perform 3D Cell Culture Media Human cancer esophageal organoids