siRNA / miRNA gene silencing Mouse MLO‐Y4

Get tips on using mTOR Antibody #2972 to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-mTOR antibody [53E11] to perform Autophagy assay cell type - HepG2

Get tips on using pASK-TS-MCoTI to perform Protein Expression Prokaryotic cells - E. coli SFTI-1

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using Phospho-mTOR (Ser2448) Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL261

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL15

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - U87MG

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,