siRNA / miRNA gene silencing Rat MTLn3 (rat mammary adenocarcinoma breast cancer cell line)

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - K562

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse brain tissue Biotin

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Gene expression arrays - Mouse dorsal skin Biotin

Get tips on using Cellstain-Double Staining Kit to perform Live / Dead assay mammalian cells - TIG-118

Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - RG2 [D74]

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - RLE-6TN

Get tips on using Anti-ATG7 antibody produced in rabbit to perform Autophagy assay cell type - UMR-106