siRNA / RNAi /miRNA transfection Rat Ar4-2j (Pancreatic tumor)

Get tips on using QuantiNova SYBR Green RT-PCR Kit (2500) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiFast Pathogen RT-PCR +IC Kit (400) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiTect SYBR Green RT-PCR Kit (1000) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Cell lines

Get tips on using Superscript reverse tran-scriptase II (SS RT II) system to perform cDNA synthesis Yeast

Get tips on using BH100bp ladder :: GD 100bp DNA Ladder H3 RTU Ladder to perform DNA Ladder 100 bp

Get tips on using QIAGEN OneStep Ahead RT-PCR Kit (200) to perform PCR Quantitative real-time PCR - Fish species DNA

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.