siRNA / miRNA gene silencing Human HT-1080

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using EpiTect PCR Control DNA Set (100) to perform PCR ORNi-PCR - Plasmid DNA

Get tips on using Strep-tag Antibody (100 ug) to perform Protein tag Detection of Strep-tagged proteins

Get tips on using Ni-NTA Superflow (100 ml) to perform Protein tag Purification of His-tagged proteins

Get tips on using RGS·His Antibody, BSA-free (100ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using mericon DNA Bacteria Kit (100) to perform DNA isolation / purification Bacteria - Gram negative Salmonella typhi

Get tips on using Bcl-2 Monoclonal Antibody (Bcl-2-100) to perform Western blotting Bcl- 2

Get tips on using RPM Yeast Plasmid Isolation Kit, 100 preps to perform Plasmid Isolation S. cerevisiae

Get tips on using IRIS9 Prestained Protein Ladder（15 to 180 kDa） to perform Protein Ladder Prestained