TissueFAxs 53BP1 [H-300]

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Get tips on using Carboxy-H2DCFDA (general oxidative stress indicator) to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Products Thermo Fisher Scientific Carboxy-H2DCFDA (general oxidative stress indicator)

Get tips on using heat shock protein family A (Hsp70) member 5 to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Products Thermo Fisher Scientific heat shock protein family A (Hsp70) member 5

Get tips on using Human HSP70 ELISA Kit to perform ELISA Human - HSP70

Products Sigma-Aldrich Human HSP70 ELISA Kit

Get tips on using HincII (HindII) restriction enzyme to perform Restriction Enzymes HincII / HindII

Products Takara Bio Inc HincII (HindII) restriction enzyme

Get tips on using HSP70 High Sensitivity ELISA kit to perform ELISA Human - HSP70

Products Enzo Life Sciences HSP70 High Sensitivity ELISA kit

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Point mutation HUVEC hCLDN5

Get tips on using HincII (HindII) (10 U/µL) to perform Restriction Enzymes HincII / HindII

Products Thermo Fisher Scientific HincII (HindII) (10 U/µL)

Get tips on using HO-1 (human), ELISA kit to perform ELISA Human - HO-1

Products Enzo Life Sciences HO-1 (human), ELISA kit

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type human umbelical vein endothelial cells (HUVEC)

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Cellular assays ROS assay cell type L-02 human fetal hepatocyte

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