Cell line authentication

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Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Proteins Protein Expression Eukaryotic cells CHO BMP-4
JetPrime Product

Get tips on using JetPrime to perform DNA transfection Mammalian cells - Primary cells Human chondrocytes

Products Polyplus transfections JetPrime

RNA siRNA / miRNA gene silencing Rat Glial cells CCR2

RNA siRNA / miRNA gene silencing Rat Schwann cells Nrf2

RNA siRNA / miRNA gene silencing Rat Schwann cells Nrp1

RNA siRNA / miRNA gene silencing Rat Schwann cells Fyn

Proteins Protein expression and purification Insect cells Sf9 Drosha

Proteins Protein expression and purification Insect cells Hi5 TYR

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