Site Directed Mutagenesis (SDM) Monkey Deletion COS-7

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using Collagen I antibody to perform Western blotting Type I collagen

Get tips on using pPICZ-C-coreTAPL to perform Protein Expression Eukaryotic cells - P. pastoris coreTAPL

Get tips on using Anti-Collagen X antibody (ab58632) to perform Immunohistochemistry Mouse - Col X

Get tips on using Anti-Collagen II antibody (ab34712) to perform Immunohistochemistry Mouse - Col II

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Get tips on using COX4 Antibody (F-8): sc-376731 to perform Western blotting COX4

Get tips on using Anti-Collagen III antibody (ab7778) to perform Western blotting Type III collagen

Get tips on using Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-