DNA transfection Mammalian cells Immortalized cell lines

Get tips on using QIAGEN Protease (30 AU) to perform Digestion during DNA preparation

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Human - MCF10A

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Mouse - EMT6

Get tips on using CometAssay Electrophoresis System II to perform DNA Damage Assay U-2 OS

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay Caco-2

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay Hep G2

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay Hep G2

Get tips on using One-step TUNEL Assay Kit to perform DNA Damage Assay HEK 293T

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.