Protein Expression Prokaryotic cells A. cellulolyticus

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell Differentiation media hTSPCs differentiation into adipogenic cells

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media hTSPCs differentiation into adipogenic cells

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell Differentiation media hMSCs differentiation into adipogenic cells

Get tips on using Dulbecco’s Modified Eagle’s Medium - low glucose to perform Stem cell Differentiation media hUMSCs differentiation into adipogenic cells

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media hUMSCs differentiation into adipogenic cells

Get tips on using TACS® 2 TdT Fluorescein Kit to perform TUNEL assay cell type - HeLa cells human cervical cancer