Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using RNA Stat-60 RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using SQSTM1/p62 (D5L7G) Mouse mAb #88588 to perform Autophagy assay cell type - MDA-MB-231

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T

Get tips on using BrdU Cell Proliferation Assay to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - OV-2008 Lipofectamine

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.