RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.
Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Salmonella enterica
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Salmonella enterica
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Pseudomonas aeruginosa
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Neisseria meningitides
Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Vibro parahaemolyticus
Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Vibrio cholera
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Haemophilus influenzae
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Staphylococcus aureus
Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Salmonella paratyphi
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment