siRNA / miRNA gene silencing Rat Brain endothelial cells

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Kupffer Cells

Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - INS-1 832/12

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using Calcein AM Cell Viability Assay Kit to perform Live / Dead assay mammalian cells - 3T3-L1

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - mouse iPSC

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - NIH/3T3

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - NIH/3T3

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - mouse keratinocytes