DNA methylation profiling Gene specific profiling MRC-5

Get tips on using REPLI-g Single Cell Kit (96) to perform Whole Genome Amplification Cell lines

Get tips on using REPLI-g UltraFast Mini Kit (100) to perform Whole Genome Amplification Cell lines

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using FastDigest MlsI to perform Restriction Enzymes MlsI / MscI

Get tips on using RNAprotect Bacteria Reagent to perform RNA stabilization Microbial

Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I

Get tips on using NucleoSpin® Plasmid to perform Plasmid Isolation Streptococcus macedonicus

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Serratia marcescens

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex