Cell migration / Invasion cell type - MHCC-97H

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Upstream tips
- Seed 2 × 10^5 cells per well in 200 μL serum-free medium.
Protocol tips
- Incubate cells for 24–48 h at 37C.

- Treat cells with 0.1 % crystal violet for 30 min, followed by washing with water for 30 s to remove residual dye
Upstream tips
Seed ~1×10^5 cells
Protocol tips
Add 20% fetal bovine serum for lower chamber

Cover plate and incubate for
12 - 24 hours at 37°C in a CO2 incubator (4-6% CO2).
Protocol tips
Incubate for 16 hours at 37ºC in 5% CO2 atmosphere
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