Wound healing assay cell type rat

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using DMEM/F12 - Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 to perform Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Get tips on using Gibco™ StemPro™ hESC SFM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using STEMdiff™ APEL™ 2 Medium to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using SIRT1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) SIRT1

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media h-medial pallium induction and culture

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media Brain organoids from Human iPSCs

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform 3D Cell Culture Media hiPSC-derived cortical organoids