Site Directed Mutagenesis (SDM) Human Point mutation A498

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Pericytes Cx43

Get tips on using Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA to perform Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1

Get tips on using IRF-1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 IRF1

Get tips on using HIF-1α siRNA (m) to perform siRNA / miRNA gene silencing Mouse - AtT20 Hif-1alpha

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - RMC-1 Cx43

Get tips on using HMG-1 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Astrocytes HMG-1

Get tips on using MEK-3 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BMDMs MEK-3

Get tips on using PU.1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 PU.1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.