Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - THP 1

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-NAPSIN A antibody (ab187300) to perform Immunohistochemistry Human - Naspsin A

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Recombinant Anti-SOX2 antibody [EPR3131] (ab92494) to perform Immunohistochemistry Human - SOX2

Get tips on using Anti-SOX9 antibody produced in rabbit to perform Immunohistochemistry Human - SOX9

Get tips on using Anti-DICER1 antibody produced in rabbit to perform Immunohistochemistry Human - Dicer1

Get tips on using Recombinant Anti-SOX9 antibody [EPR14335] (ab185230) to perform Immunohistochemistry Rat - Sox9

Get tips on using Recombinant Anti-PRMT5 antibody [EPR5772] (ab109451) to perform Immunohistochemistry Rat - PRMT5

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.