Stem cell culture media

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media PNT1A

Get tips on using CD CHO Medium to perform Mammalian cell culture media CHO

Get tips on using HEK GM to perform Mammalian cell culture media HEK

Get tips on using Advanced DMEM to perform Mammalian cell culture media HC

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

Get tips on using Keratinocyte-SFM to perform Mammalian cell culture media HaCaT

Get tips on using RPMI 1640 to perform Mammalian cell culture media A2780

Get tips on using RPMI 1640 to perform Mammalian cell culture media CHO

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hUMSCs differentiation into steroidogenic cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.